GE Healthcare ECL plus Manual
Anti rabbit IgG, peroxidase linked whole antibody (from donkey) 100. μ. l. ECL. TM. Membrane blocking agent. 5g. Detection of membrane bound primary antibodies . ->
Convert to SuperSignal West Pico Substrate from ECL Substrate
Conditions given for ECL Substrate are those suggested by the manufacturer. Antibody dilutions . require much less secondary antibody than. ECL Substrate. . ->
Western Blotting Handbook and Troubleshooting Guide
lotting because an antibody is used to speci? cally detect its . primary antibody dilution from a 1 mg/ml stock = 2 μg/ml with an ECL Substrate. . ->
Kamps's Western Protocol
ECL is an appealing technique because it is quick and very sensitive and does . ECL analysis of immunoprecipitates using a second antibody is almost always . ->
ECL Plus Western Blotting Detection Reagents
antibodies) ECL Blocking Agent (RPN2125) Description. The ECL Plus Western blotting detection . secondary antibodies are required with ECL Plus compared to ECL . ->
GE Healthcare Life Sciences - Products - Chemiluminescent Labeling and .
Amersham? ECL Advance? enables detection also with low antibody concentrations . Amersham ECL?-HRP Linked Secondary Antibodies. Highly species-specific. . ->
Phosphotyrosine Western Blotting
. a different antibody under identical conditions using ECL technology (GE . with PY20 antibody, and electronic photos of stained blot and ECL film. . ->
Phosphoserine and phosphothreonine Western Blotting
The ultra sensitive ECL Advance enables us to dilute the antibodies to 1:4000 . combined Q5/Q7 antibodies, and electronic photos of stained blots and ECL films. . ->
Sheep Anti-Mouse Secondary Antibodies (Horseradish Peroxidase .
Sheep Anti-Mouse Secondary Antibodies (Horseradish Peroxidase Conjugated) . Sheep Anti-Mouse IgG, Whole Ab ECL Antibody, HRP Conjugated . ->
Western Blot - R&D Systems
Problem: White Bands (ECL method) Problem: Patchy uneven spots all over the blot . Increase antibody concentration (2-4 fold higher than recommended starting . ->
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